When applying to register a copyright, do I need to submit a copy of my work?

No, the Copyright Office does not accept copies of a work, however Canadian works sometimes need to be deposited at Library and Archives Canada. Please contact Library and Archives Canada for more information:

Library and Archives Canada
Legal Deposit
395 Wellington Street
Ottawa, Ontario
K1A 0N4
Tel.: 1-866-578-7777 or 819-997-9565
613-996-5115
Fax: 613-995-6274
Website: http://www.collectionscanada.gc.ca/index-e.html